The Cytobank platform has comprehensive gating tools for data analysis. Here, we will give an overview of gating and gating-based visualization in the Cytobank platform. Please see The Difference Between a Gate and a Population to learn more about how to create populations using gates.
In the Gating interface anatomy section, you can click on each item to go to the dedicated support article about the gating tools to help you navigate.
For a complete overview of gating in the Cytobank platform, see our video below!
Navigating to the Gating Editor
From within an experiment, click on Gates>Gating Editor in the experiment navigation bar.
The Gating Editor is where you can create gates and populations and visualize them. In the Gating hierarchy you can view the gating hierarchy by files and population.
Gating interface anatomy
Please click on the highlighted text to learn more in the dedicated support article about all the gating tools.
(1) Apply gates
To apply the current state of gates and populations to the rest of the analysis components like illustrations and advanced analyses.
(2) Gating tools
To choose between Rectangle, Elipse, Polygon, Quadrant, Split and Range gate tools to draw gate around cell populations.
(3) Import gates
To import gates to a current experiment, from another experiment or by uploading gating-ML.
(4) Cluster gates
To create cluster gates with cluster channels.
(5) Reset all
To remove all gates and populations from the gating interface. This action cannot be undone.
To choose the compensation to use for the Gating Editor.
To show a list of the gates created. Gates are the coordinates of the X and Y channels and do not carry hierarchical information. Gates can be used to build hierarchy to define a population.
To show the current active population displayed in the plot. The up and down arrows to the right can be used to move up or down the population list.
To show the current active file displayed in the plot. The left and right arrows to the right can be used to move up or down the file list.
(10) Plot settings:
To change the plot settings.
Click on the setting icon to show the Plot setting window. Please see the link for more information on the Colored by Channel feature.
(11) Population tree:
List view of population hierarchy.
(12) Boolean expressions:
To view and create populations by Boolean expression.
(13) Sunburst view:
Sunburst view of the population hierarchy.
*For Research Use Only. Not for use in diagnostic procedures.