In most Cytometry software, data are written in uncompensated format and the compensation matrix is included within the files alongside the data. This internal instruction for compensation is called a "file-internal compensation" and is typically held in the FCS variables of SPILL or $SPILLOVER. In Cytobank, use the file-internal option whenever choosing a compensation to have your data automatically compensated on a per-file basis according to the internal instructions. Data can still be viewed as uncompensated by changing the compensation selector to "uncompensated"
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