Many users choose to calculate a compensation matrix directly on the flow cytometer prior to collecting samples. However, sometimes it becomes apparent during analysis this computed matrix needs adjustment. To modify file-internal compensation, you first need to extract the matrix.Access the Compensation page by clicking the "Comps" button on the experiment level navigation bar.
Under the "File-Internal Compensation" tab, select a representative FCS file that contains the file-internal compensation matrix and click "Import Compensation". The file-internal compensation matrix values will appear. This matrix will now appear under the list of compensations that you can select in the Working Illustration and Gating Interface.
(electing to import a file-internal matrix from a file)
To make edits, click inside any cell with a number and edit that numerical value. Changes will be automatically saved and reflect in the plot to the left.
(adjusting a compensation matrix manually)