How to show gates on illustration plots

Remember that a gate is tied to a compensation matrix and channel selection and is visible only when they are set accordingly. If gates cannot be seen in an illustration, the issue can be remedied with instructions provided in the above article. 

Gates must be applied before they will show in the Illustration. Please click on Apply gates in the Gating editor before configuring an Illustration.

To configure gates to display on dot or contour plots in an Illustration, turn on the Show gates toggle from the Plots menu.

Check the gating interface to ensure the gate is drawn on the channels and settings that you expect. Next, you have the option to display your desired Gate Statistic: None, Percentages, Event Count, or Gate Name from the Gate label setting on the Plots menu.


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When using concatenated dot/contour plots with tailored gate per file, the gate position displayed on the plot will be the one of the first file in the concatenated plot. Event counts and Percent in gate display statistics based on the number of concatenated events.

When using colored overlay dot plots, gates can be shown but their statistics will not be shown.

- If tailored gate per file was selected, gate position is same as concatenated plot. 

-If the populations are overlaid, the position of the gate will change per file according to what was set in the Gating editor. 

-If the files or sample tags are overlaid, then the position of the first file will display.

Create a support request to alert us if this is important for your workflow.


*For Research Use Only. Not for use in diagnostic procedures.

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