Depending on the file, it isn't rare that a gate will have to be placed in a different location across a set of FCS files. To complete this step, you will need to use tailored gates per file.
While tailoring gates, it is usually helpful to be able to see the location that the gate falls across all files. You can use the Check Gates functionality for this.
How to use Check Gates
To use Check Gates, select a gate by clicking on it in the Gating Editor plot or in the list of gates on the left of screen. Next, simply press the Check Gates icon: this will open a new tab and view the gate location across all files in the experiment. Check Gates will show you events on plots based on the active population, compensation, plot type and plot color, that are currently selected in the gating interface.
The result of Check Gates
(Click to expand - how to use Check Gates to see gate location on all samples)
*For Research Use Only. Not for use in diagnostic procedures.