Can't see a gate? In the Cytobank platform, a gate shows on a plot only when these conditions are met:
- The compensation that the gate was drawn on is selected.
- The channels that the gate was drawn on are selected.
In the Gating Editor, if the compensation that the gate was drawn on is different from the applied experiment-wide compensation in the experiment, you will see an eye-backslash icon next to the gate name in the list of Gates. The icon beside the gate name stands for Hidden.
Clicking on the hidden gate in the list of Gates will show a warning message of compensation difference. To see the gate on the plot and change the applied experiment-wide compensation to the one on which the gate was drawn, click the compensation red box on the top right. Please check this article to know more about the impact of changing the compensation matrix on already drawn gates.
If the channel(s) that the gate was drawn on is different or not included in the FCS files in the Experiment, you also will see the hidden icon in the list of Gates. The settings on which the gate was drawn can be viewed by hovering the mouse over the gate name in the list of gates and “undefined for current Panel” indicates which axis does not match the channel between setting and current.
The same principles apply to making a gate appear on a plot in the illustration.
Have more questions? Submit a request.
*For Research Use Only. Not for use in diagnostic procedures.