Can't see a gate? In Cytobank, a gate only shows on a plot when three conditions are met:
- The channels that the gate was drawn on are selected.
- The compensation that the gate was drawn on is selected.
- The scale settings for the channels that the gate was drawn on are set as they were when the gate was drawn.
In the gating interface, if your compensation or scales are set differently than a gate expects them, you will see an (H) next to the gate name in the list of gates. The H beside the gate name stands for "Hidden".
Clicking on the gate in the list of gates will snap the selected compensation to the one on which the gate was drawn and show the gate. Scales, however, have to be changed manually to make the gate appear again. The settings on which the gate was drawn can be viewed by hovering the mouse over the gate name in the list of gates.
The same principles apply to making a gate appear on a plot in the working illustration.
Contact Cytobank Support for a special command that allows your gates to be redefined by your current scale settings, and thus appear.