What is SPADE?

SPADE stands for "Spanning-tree Progression Analysis of Density-normalized Events." SPADE clusters phenotypically-similar cells into a hierarchy that allows high-throughput, multidimensional analysis of heterogeneous samples. Read more about it in this publication by the Nolan Lab:

Extracting a cellular hierarchy from high-dimensional cytometry data with SPADE. Qiu P, et al. Nat Biotechnol (2011) 29(10):886-91

You can get started running a SPADE analysis on your data on hosted models of Cytobank. Navigate to the Experiment Details page for your experiment, and click the SPADE link on the left.

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